Isolation and properties of an RNA fraction present inBrucellaculture supernatants

Abstract

SUMMARYThe supernatant fluids of batch and continuous cultures ofBrucellastrains contained up to 100 mg/i of soluble RNA which could be recovered by precipitation with lysozyme. This RNA fraction had many of the properties of ribosomal RNA and was single-stranded, sensitive to ribonuclease, with an approximate sedimentation constant of 5S, a molecular weight of about 35000 daltons and an adenine; guanine; cytosine; uracil content of 17·5 26·5 33; 23 mol% respectively. RNA fractions from lysozyme precipitates evoked high titres ofBrucellaagglutinins on injection into rabbits and induced acute inflammatory responses in guinea-pig skin. Highly purified RNA fractions prepared by phenol extraction of lysozyme precipitates did not evoke antibodies toBrucella abortus.