The effect of different extraction procedures on the recovery of cell walls in forage and faeces from cattle and sheep

Author:

Moir K. W.

Abstract

SUMMARYForages and faeces from 28 digestibility experiments with cattle or sheep were extracted with neutral detergent solution, with and without sodium sulphite, or sequentially extracted with acid-pepsin, ethyl alcohol, diethyl ether and either ammonium oxalate or hot water. Ammonium oxalate was used to extract small amounts of non-protein material not extracted from legumes by hot water. Compared with sequential extraction the average protein-free organic residues after extraction with detergent, with and without sodium sulphite, were significantly lower in legumes and faeces, but not in grasses. The largest differences occurred in faeces from grasses for which the recoveries of cell walls as percentages of the faecal organic matter were found to be 59·2, 62·0 and 66·4 respectively, after extraction with neutral detergent plus sodium sulphite, neutral detergent alone and sequential extraction with various solvents. It was considered that the differences were due to extraction of cell-wall constituents by both detergent and sulphite.

Publisher

Cambridge University Press (CUP)

Subject

Genetics,Agronomy and Crop Science,Animal Science and Zoology

Reference7 articles.

1. Van Soest P. J. , Wine R. H. & Moore L. A. (1966). Estimation of the true digestibility of forages by the in vitro digestion of coll walls. Proc. X int. Grassld Congr., pp. 438–41.

2. The evaluation of artificially dried grass as a source of energy for sheep:I. The effect of stage of maturity on the apparent digestibility of rye-grass, cocksfoot and timothy

3. Use of detergents in the analysis of fibrous feeds. IV. Determination of plant cell-wall constituents;Van Soest;J. Ass. off. analyt. Chem.,1967

4. Van Soest P. J. & Moore L. A. (1965). New chemical methods for analysis of forage for the purpose of predicting nutritive value. Proc. IX int. Grassld Congr., pp. 785–9.

5. The comprehensive analysis of grasses

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