Efficacy of rhizobacteria for growth promotion in sorghum under greenhouse conditions and selected modes of action studies

Abstract

SUMMARYThe screening of rhizobacteria for growth promotion of sorghum (Sorghum bicolour(L.) Moench) was conducted under greenhouse conditions for a total of 78 bacteria isolated from the rhizosphere of sorghum in Ethiopia, and 86 isolates from the rhizosphere and rhizoplane of grasses in South Africa. Three isolates from Ethiopia, all identified asBacillus cereus, enhanced growth promotion by resulting in statistically significant increases in at least five parameters. Of these,B. cereus(KBE7-8) resulted in significant increase in shoot and root biomass. Among effective isolates from South Africa,B. cereus(NAS4-3) andStenotrophomonas maltophilia(KBS9-B) showed significant increases in all the parameters measured. The isolates which resulted in significant growth promotion colonized the roots effectively with a count up to ⩾108cfu/g. In the study conducted to elucidate the possible modes of action by these effective isolates, indole 3-acetic acid-like substances were detected in culture filtrates of the isolates ranging from 4·2 μg/ml bySerratia marcescens(KBS9-R) to 22·8 μg/ml byB. cereus(KBS5-H) in the presence of 2 mg tryptophan/g nutrient broth solution. Higher rates of solubilization of tricalcium phosphate on Pikovskaya agar medium were shown byChryseomonas luteola(KBS5-F),S. marcescens(KBS6-H) andB. cereus(KBE9-1). There is very limited knowledge of the use of rhizobacteria in agriculture in Ethiopia and South Africa. The current study therefore generates valuable information towards application of plant growth promoting rhizobacteria as alternatives to chemical fertilizers.