Cryo-Fracture or Freeze-Fracture, a Method to Expose Internal Tissue Surfaces and Cell Surfaces for Viewing in the Scanning Electron Microscope

Author:

Taylor Jeannette

Abstract

Cryo-fracture, in conjunction with critical point drying is a method used to prepare biological samples in order to expose, for viewing via scanning electron microscopy, those naturally occurring surfaces which might otherwise remain obscure. For example, the Bowman’s capsule and tubules of a kidney, tiny blood vessels on any organ, inter-cellular spaces in liver or alveoli in the lungs. Also, some surfaces, not normally exposed at all such as the membrane surface of a nuclear envelope, mitochondria or chloroplasts or the cytoplasm of a cell, can be brought to light with this method. Herein is a review of the development of cryo-fracture and how it is currently used at our facility.

Publisher

Cambridge University Press (CUP)

Subject

General Medicine

Reference7 articles.

1. Apkarian R. & Curtis J. C. , “SEM Cryofracture Study of Ovarian Follicles of Immature Rats”, Scanning Electron Microscopy, Vol. VI, 1981, pp. 165-172

2. Humphreys Walter J. , Spurlock Ben O. , & Johnson Janet S. , “Transmission Electron Microscopy of Tissue Prepared for Scanning Electron Microscopy by Ethanol- Cryofracture”, Stain Technology, 1975, pp. 119-125

3. Haggis G. H. , “Cryofracture of Biological Material”, Scanning Electron Microscopy, April, 1970, pp. 97-104 *Though sucrose is an osmotic agent rather than a buffering agent, in his report, Dr. Haggis’ indeed describes his fixative as “2% glutaraldehyde in sucrose buffer at pH 7.4”.

4. Humphreys Walter J. , Spurlock Ben O. , & Johnson Janet S. , “Critical Point Drying of Freeze-Fractured Tissue for Scanning Electron Microscopy”, Proceeding of Thirty-First Annual EMSA Meeting, 1973, pp. 452-453

5. Hotchkiss Anne E. , Martin Vicki J. , & Apkarian Robert P. , “A Scanning Electron Microscopic Surface and Cryofracture Study of Development in the Planulae of the Hydrozoan, Pennaria tiarella”, Scanning Electron Microscopy, Vol. II, 1984, pp. 717-727.

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