Abstract
Attempts to fix the gregarinePterospora floridiensis(a protozoan parasite found in marine bristle worms) for TEM using a conventional glutaraldehyde and osmium protocol produced poor preservation of the outer pellicular membranes and internal membranous structures. However, fixation success was achieved using the well-known fixative additives tannic acid and potassium ferrocyanide, which improved membrane preservation, cytoplasmic integrity, and contrast in both the unencysted feeding stage ofP. floridiensisas well as the difficult-to-fix cyst stage. The longer fixation times (24 hours) needed for the cysts were compatible with the modified fixatives. Interestingly,bothadditives were needed to improve fixation.
Publisher
Cambridge University Press (CUP)
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