Author:
Nikishin Denis A.,Milošević Ivan,Gojković Milorad,Rakić Ljubisav,Bezuglov Vladimir V.,Shmukler Yuri B.
Abstract
SummaryReverse-transcription polymerase chain reaction (RT-PCR) investigation of the expression of the components supposedly taking part in serotonin regulation of the early development ofParacentrotus lividushas shown the presence of transcripts of five receptors, one of which has conservative amino acid residues characteristic of monoaminergic receptors. At the early stages of embryogenesis the expressions of serotonin transporter (SERT) and noradrenaline transporter (NET) were also recognized. The activities of the enzymes of serotonin synthesis and serotonin transporter were shown using immunohistochemistry and incubation with para-chlorophenylalanine (PСРА) and 5-hydroxytryptophan (HTP). Pharmacological experiments have shown a preferential cytostatic activity of ligands characterized as mammalian 5-hydroxytryptamine (5-HT)1-antagonists. On the basis of the sum of the data from molecular biology and embryo physiological experiments, it is suggested that metabotropic serotonin receptors and membrane transporters take part in the regulatory processes of early sea urchin embryogenesis.
Publisher
Cambridge University Press (CUP)
Subject
Cell Biology,Developmental Biology
Reference42 articles.
1. Molecular architecture of G protein-coupled receptors
2. Sensitivity of fragmented and stratified sea urchin embryos to cytotoxic neuropharmacological preparations;Buznikov;Russ. J. Dev. Biol.,1979
3. The role of neurohumours in early embryogenesis.: I. Serotonin content of developing embryos of sea urchin and loach;Buznikov;J. Embryol. Exp. Morphol,1964
4. International Union of Pharmacology classification of receptors for 5-hydroxytryptamine (serotonin);Hoyer;Pharmacol. Rev.,1994
5. Principles governing amino acid composition of integral membrane proteins: application to topology prediction 1 1Edited by J. Thornton
Cited by
11 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献