Quantitative analysis of Protective Role of salivary protein on demineralization of enamel

Abstract

Objective: The aim of this study was to analyze the effect of salivary proteins (statherin and histatin-1) on demineralized enamel surface and to study changes in texture, magnitude and direction of crystallites and changes in prismatic structure of enamel respectively. Material & Methods: Synchrotron x-ray diffraction technique to determine the variation in degree of crystal orientation (texture). Incisors were demineralized and sectioned to 300-500 microns, rinsed with salivary protein solutions of statherin and histatin separately and in combination with short and full-lengths. A beam spot size of 20μm × 20μm was used to obtain 2D diffraction patterns to distinguish orientation of crystallites. Results: The contour maps as well as the SEM analysis present similar surface properties of the sample treated with STN-21 and the controlled PBS sample. Therefore, STN-21 was found potent in preventing demineralization and restoring surface enamel texture followed by STN-21+HTN-21 and STN43+HTN38. HTN-21 and HTN-38 showed similar demineralization pattern as the controlled demineralized sample. Conclusion: Ranking of demineralization among samples was found to be controlled demineralized > HTN-21 = HTN-38 > STN-43+HTN-38 > STN-21+HTN-21 > STN-21. Developing STN-21 as a therapeutic against dental caries and erosion. Keywords: Enamel, Demineralization, salivary proteins.