Protective Effect of Chichorium Intybus (Kasni) Roots on Hepatotoxocity Induced By Pyrazinamide in Male Mice

Abstract

Background: Tuberculosis (TB) is major health issue across the world. Yearly 2 million deaths are reported from this disease. Pakistan has been ranked in the top six countries that are bearing the major brunt of TB. Aim: To observe hepatoprotective role of aqueous extract of kasni (Chichorium intybus) roots on hepatotoxicity caused by Pyrazinamide in male BALB/c mice. Study Design: Randomized control Trial. Methodology: Healthy male albino Balb/C mice, fifty six in number were selected randomly and further grouped into four groups (n=14 mice). Group A labelled as control group and maintained on rodent diet with no medication. Group B was labelled as disease control group and only pyrazinamide was administered to mice in this group in dose (500mg/kg BW). Groups C and D designated as exploratory groups. Both group C & D were administered a combination of Anti TB drug, pyrazinamide along with aqueous extract preparations of Kasni roots in low (200mg/kg) & high doses (400mg/kg) respectively. Baseline blood samples were drawn at day zero. Results: Serum alanine aminotransferase (ALT) and alkaline Phosphatase (ALP) were improved in group C & D given aqueous extract of Chicorium intybus roots. Conclusion: It was concluded that high doses of aqueous extract preparations of kasni (Chichorium intybus) roots showed more improvements of serum markers in drug induced hepatotoxicity than low doses of aqueous preparations. Keywords: Chichorium intybus, Hepatoprotective, Alanine aminotransferase (ALT), Alkaline Phosphatase (ALP),