SODIUM ALGINATE/PRP MATRICES FOR PROSTAGLANDIN RELEASE IN ACUTE MYOCARDIAL INFARCTION

Abstract

Tissue engineering is a multidisciplinary field, and biocompatibility, biodegradability, shear behavior, rapid gelation, and an easy cross-linking process make alginate one of the most extensively studied polysaccharides in this area. Myocardial infarction represents the leading cause of death worldwide. Recently, peroxisome proliferator-activated receptors (PPARs) have garnered research interest due to their role in modulating inflammation. 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) has demon-strated significant antinociceptive and anti-inflammatory activity. In the pursuit of a shorter and less invasive drug delivery system, a biodegradable matrix was sought. Leveraging the characteristics of alginate and platelet-rich plasma (PRP) as biode-gradable biomaterials with suitable physicochemical and biological properties for this application, this study aimed to develop an alginate and PRP biomaterial for prosta-glandin release. Platelet-Rich Plasma (PRP) was obtained by concentrating platelets through centrifugation. The biomaterial, in the form of membranes, was prepared using the casting method from polymeric solutions with a concentration of 4.0% (w/v), with PRP added in proportions of 10% relative to the mass of sodium alginate (SA). The preformed membranes were immersed in aqueous solutions of 1% CaCl2 (w/v) to promote SA cross-linking and subsequent drug incorporation. In vitro bioac-tivity was evaluated by immersion in simulated body fluid. The SEM assay demon-strated that the incorporation of SA/PRP/PGJ occurred uniformly. Therefore, the results suggest the potential use of alginate and PRP films for prostaglandin release, but pre-clinical studies are still required.