Abstract
ObjectivesIslet transplantation is an emerging treatment option for type 1 diabetes but its application is limited by the shortage of human pancreas donors. Characterization of theN- andO-glycan surface antigens that vary between human and genetically engineered porcine islet donors could shed light on targets of antibody mediated rejection.MethodsN- andO-glycans were isolated from human and adult porcine islets and analyzed using matrix-assisted laser-desorption time-of-flight mass spectrometry (MALDI-TOF-MS) and electrospray ionization mass spectrometry (ESI-MS/MS).ResultsA total of 57 porcine and 34 humanN-glycans and 21 porcine and 14 humanO-glycans were detected from cultured islets. Twenty-eight of which were detected only from porcine islets, which include novel xenoantigens such as high-mannose typeN-glycans with core fucosylation and complex-typeN-glycans with terminal neuraminic acid residues. Porcine islets have terminalN-glycolylneuraminic acid (NeuGc) residue in bi-antennaryN-glycans and sialyl-TnO-glycans. No galactose-α-1,3-galactose (α-Gal) or Sdaepitope were detected on any of the islets.ConclusionsThese results provide important insights into the potential antigenic differences ofN- andO-glycan profiles between human and porcine islets. Glycan differences may identify novel gene targets for genetic engineering to generate superior porcine islet donors.
Publisher
Public Library of Science (PLoS)
Cited by
14 articles.
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