Abstract
Leptospirosis is an important global zoonosis caused by pathogenic Leptospira. It is estimated that more than 1 million people are infected by Leptospira each year, and the death toll is about 60,000. Some studies showed that delayed immune response was associated with severe leptospirosis, and TLR4 was very important in the control of leptospirosis. In this study, we aimed to explore the effect of the classical activator (LPS) of TLR4 on leptospirosis in susceptible and resistant hosts. The results showed that LPS pretreatment increased the survival rate of hamsters to 80%. And LPS pre-treatment also significantly reduced the leptospiral load and alleviated the pathological injury in organs of hamsters and mice. The result detected by ELISA in mice showed that the levels of TNF-α and IL-1β were increased in the LPS-treated group compared to the control group before infection. However, two days after infection, the level of cytokines in LPS group was down-regulated compared with that in control group. In addition, in vitro results showed that LPS pre-treatment enhanced the phagocytosis and bactericidal ability of macrophages on Leptospira. Collectively, our results indicated that the pre-activated immune response induced by LPS enhanced the ability of host against leptospirosis.
Funder
National Natural Science Foundation of China
Key Project of Chinese National Programs for Research and Development
Publisher
Public Library of Science (PLoS)
Reference35 articles.
1. Leptospirosis: a silent epidemic disease;MC Schneider;International journal of environmental research and public health,2013
2. Leptospiral membrane proteins stimulate pro-inflammatory chemokines secretion by renal tubule epithelial cells through toll-like receptor 2 and p38 mitogen activated protein kinase;CC Hung;Nephrology, dialysis, transplantation: official publication of the European Dialysis and Transplant Association—European Renal Association,2006
3. Leptospirosis: a zoonotic disease of global importance;AR Bharti;The Lancet. Infectious diseases,2003
4. Leptospirosis in humans;DA Haake;Current topics in microbiology and immunology,2015
5. Leptospirosis: Increasing importance in developing countries;ZMP Soo;Acta tropica,2020
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