Automatic reagent handling and assay processing of human biospecimens inside a transportation container for a medical disaster response against radiation

Author:

Akkad Adam R.,Gu JianORCID,Duane Brett,Norquist Alan,Brenner David J.,Ramakumar Adarsh,Zenhausern Frederic

Abstract

Biological materials can be shipped off-site for diagnostic, therapeutic and research purposes. They usually are kept in certain environments for their final application during transportation. However, active reagent handling during transportation from a collection site to a laboratory or biorepository has not been reported yet. In this paper, we show the application of a micro-controlled centrifugal microfluidic system inside a shipping container that can add reagent to an actively cultured human blood sample during transportation to ensure a rapid biodosimetry of cytokinesis-block micronucleus (CBMN) assay. The newly demonstrated concept could have a significant impact on rapid biodosimetry triage for medical countermeasure in a radiological disaster. It also opens a new capability in accelerated sample processing during transportation for biomedical and healthcare applications.

Funder

Armed Forces Radiobiology Research Institute, Uniformed Services University

Congressionally Directed Medical Research Programs

Publisher

Public Library of Science (PLoS)

Subject

Multidisciplinary

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