Cell tip growth underlies injury response of marine macroalgae

Abstract

Regeneration is a widely observed phenomenon by which the integrity of an organism is recovered after damage. To date, studies on the molecular and cellular mechanisms of regeneration have been limited to a handful of model multicellular organisms. Here, the regeneration ability of marine macroalgae (Rhodophyta, Phaeophyceae, Chlorophyta) was systematically surveyed after thallus severing. Live cell imaging on severed thalli uncovered the cellular response to the damage. Three types of responses–budding, rhizoid formation, and/or sporulation–were observed in 25 species among 66 examined, proving the high potential of regeneration of macroalgae. The cellular and nuclear dynamics were monitored during cell repair or rhizoid formation of four phylogenetically diverged species, and the tip growth of the cells near the damaged site was observed as a common response. Nuclear translocation followed tip growth, enabling overall distribution of multinuclei or central positioning of the mononucleus. In contrast, the control of cell cycle events, such as nuclear division and septation, varied in these species. These observations showed that marine macroalgae utilise a variety of regeneration pathways, with some common features. This study also provides a novel methodology of live cell imaging in macroalgae.