Abstract
Background
Human immunodeficiency virus type 1 (HIV-1) genetic diversity and pre-treatment drug resistance (PDR) are major barriers to successful antiretroviral therapy (ART). In China, sexual intercourse is the most frequent route of HIV-1 transmission. However, few studies have analyzed PDR and transmission networks in detail among individuals in China with acute HIV-1 infection and their sexual contacts.
Methods
A cross-sectional study was conducted in Baoding City, Hebei Province, China from 2019–2020. CD4 T cell counts and viral loads were assessed and a HIV-1 genotypic PDR assay was developed in-house. Transmission networks were visualized using Cytoscape with a threshold genetic distance of 0.015 among HIV-1 subtypes.
Results
From 139 newly diagnosed and drug-naïve individuals with HIV-1, 132 pol gene sequences were obtained and revealed eight HIV-1 subtypes. Circulating recombinant form (CRF)01_AE was the most frequent subtype (53.0%, 70/132) followed by CRF07_BC (26.5%, 35/132), B (13.6%, 18/132), unique recombinant forms (2.3%, 3/132), CRF55_01B (1.5%, 2/132), CRF103_01B (1.5%, 2/132), CRF65_cpx (0.8%, 1/132), and C (0.8%, 1/132). A total of 47 pol gene sequences were used to generate 10 molecular transmission networks. The overall prevalence of PDR was 7.6% and that of PDR to non-nucleotide reverse transcriptase inhibitors was 6.1%. Of three transmission networks for PDR, two were closely associated with Beijing and Tianjin, while another was restricted to sequences determined in this study.
Conclusions
These results demonstrate that during acute HIV-1 infection, PDR is transmitted in dynamic networks. This suggests that early detection, diagnosis, surveillance, and treatment are critical to effectively control HIV-1 spread.
Funder
the Science and Technology Planning Project of Baoding Prefecture
Publisher
Public Library of Science (PLoS)
Reference23 articles.
1. 2021 Progress Report on the Global AIDS response;UNAIDS
2. Current status and prospects of HIV treatment;C Tomas;Current Opinion in Virology,2016
3. Review: Influence of ART on HIV genetics;RS Francesco;Curr Opin HIV AIDS,2015
4. Effect of highly active anti-retroviral therapy on reducing HIV/AIDS related death in Hebei, 1989–2013.;S Chen;Chin J Epidemiol,2015
5. HAART in HIV/AIDS treatments: future trends.;DY Lu;Infectious Disorders-Drug Targets,2018
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