Abstract
Xylanase is one of industrial enzymes with diverse applications including the paper-bleaching industry and feed additives. Here, a strain having xylanolytic activity and identified as Bacillus sonorensis T6 was isolated from soil. A secretory enzyme was identified by mass-spectrometry as a xylanase of glycosyl hydrolase family 11, with a molecular weight of 23.3 kDa. The xylanase gene of Bacillus sonorensis T6 was cloned and expressed in Escherichia coli (yielding an enzyme designated as rXynT6-E) and in Pichia pastoris (yielding rXynT6-P). The recombinant xylanases were found to have optimal activity at 47–55°C and pH 6.0–7.0. The recombinant xylanase expressed in P. pastoris has 40% higher thermal stability than that expressed in E. coli. The recombinant xylanases retained 100% of activity after 10 h incubation in the pH range 3–11 and 68% of activity after 1 h at pH 2.0. The xylanase activities of rXynT6-E and rXynT6-P under optimal conditions were 1030.2 and 873.8 U/mg, respectively. The good stability in a wide range of pH and moderate temperatures may make the xylanase from Bacillus sonorensis T6 useful for various biotechnological applications, e.g., as an enzyme additive in the feed industry.
Funder
Science Committee of the Ministry of Education and Science of the Republic of Kazakhstan
Publisher
Public Library of Science (PLoS)
Reference55 articles.
1. Wood Hemicelluloses: Part I
2. Microbial xylanases: engineering, production and industrial applications;V Juturu;Biotechnology advances,2012
3. Structural chemistry of the hemicelluloses;GO Aspinall;Advances in carbohydrate chemistry,1959
4. Microbial xylanase: A review.;A Popa;Scientific Bulletin Series F Biotechnologies,2016
5. Production, characteristics, and biotechnological applications of microbial xylanases.;Singh B Alokika;Applied microbiology and biotechnology,2019
Cited by
12 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献