A simulation-based assessment of the efficiency of QTL mapping under environment and genotype x environment interaction effects

Abstract

The objective of this simulation-based study was to assess how genes, environments, and genotype x environment (GxE) interaction affect the quantitative trait loci (QTL) mapping efficiency. The simulation software performed 50 samplings of 300 recombinant inbred lines (RILs) from a F2, which were assessed in six environments. The RILs were genotyped for 977 single nucleotide polymorphisms (SNP) and phenotyped for grain yield. The average SNP density was 2 cM. We defined six QTLs and 190 minor genes. The trait heritability ranged from 30 to 80%. We fitted the single QTL model and the multiple QTL model on multiple phenotypes. The environment and complex GxE interaction effects led to a low correlation between the QTL heritability and power. The single- and across-environment analyses allowed all QTLs be declared, with an average power of 28 to 100%. In the across-environment analysis, five QTLs showed average power in the range 46 to 82%. Both models provided a good control of the false positive rate (6%, on average) and a precise localization of the QTLs (bias of 2 cM, on average). The QTL power in each environment has a high positive correlation with the range between QTL genotypes for the sum of the additive, environment, and GxE interaction effects (0.76 to 0.96). The uncertainty about the magnitude and sign of the environment and GxE interaction effects makes QTL mapping in multi-environment trials unpredictable. Unfortunately, this uncertainty has no solution because the geneticist has no control over the magnitude and sign of the environment and GxE interaction effects. However, the single- and across-environment analyses are efficient even under a low correlation between QTL heritability and power.