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Target discovery screens using pooled shRNA libraries and next-generation sequencing: A model workflow and analytical algorithm

  • Published:2018-01-31 Issue:1 Volume:13 Page:e0191570
  • ISSN:1932-6203
  • Container-title:PLOS ONE
  • language:en
  • Short-container-title:PLoS ONE

Author:

Schaefer Christiane,Mallela Nikhil,Seggewiß Jochen,Lechtape Birgit,Omran Heymut,Dirksen Uta,Korsching Eberhard,Potratz JennyORCID

Funder

Bundesministerium für Bildung und Forschung

European Commission FP7

Medizinische Fakultät, Westfälische Wilhelms-Universität Münster

IMF, Medizinische Fakultät, Westfälische Wilhelms-Universität Münster (DE)

Deutsche Krebshilfe

Publisher

Public Library of Science (PLoS)

Subject

Multidisciplinary

Reference38 articles.

1. Cancer proliferation gene discovery through functional genomics;MR Schlabach;Science,2008

2. Essential gene profiles in breast, pancreatic, and ovarian cancer cells;R Marcotte;Cancer Discov,2012

3. A large-scale RNAi screen in human cells identifies new components of the p53 pathway;K Berns;Nature. Nature Publishing Group,2004

4. Genome-wide functional screen identifies a compendium of genes affecting sensitivity to tamoxifen;AM Mendes-Pereira;Proc Natl Acad Sci USA,2012

5. Synthetic lethal screen identification of chemosensitizer loci in cancer cells;AW Whitehurst;Nature,2007

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