Abstract
Cowpea [Vigna unguiculata (L.) Walp.] is a crop with significant agronomic and nutritional value. In Togo, the crop is very appreciated by local people. It is the third food habit in Togo after maize and rice. However, several accessions of cowpea cultivated in Togo are now prone to extinction, creating a risk of genetic erosion. It is therefore urgent to assess the genetic diversity of accessions in order to set up a good conservation program. To achieve this, genetic diversity and phylogenetic relationships among 70 accessions of cowpea collected in the five (5) administrative regions of Togo were assessed using Simple Sequence Repeat (SSR) molecular markers. The twenty-eight SSR primers used in this study generated a total of 164 alleles with an average of 5.82 alleles per locus. Polymorphic Information Content (PIC) values ranged from 0.20 to 0.89 with an average value of 0.58. Population structure analysis using model-based revealed that the cowpea germplasm was grouped into two subpopulations. The analysis of molecular variance (AMOVA) revealed that 98% of genetic variation existed among accessions within regions. The fixation index (Fst) value, which was 0.069 was low, indicating relatively low population differentiation. The phylogenetic analysis grouped the 70 accessions into two main groups that can be further divided into four groups independent of their origins. This study provides a foundation for a Togolese cowpea germplasm conservation program and can serve for the selection of parental material for further studies aimed at the genetic improvement of local germplasm.
Funder
International Foundation for Science
Publisher
Public Library of Science (PLoS)
Reference48 articles.
1. Recent advances in cowpea breeding. Advances in cowpea research. Co-publication of International Institute of Tropical Agriculture (IITA) and Japan International Research Center for Agricultural Sciences (JIRCAS);BB Singh;Ibadan. Nigeria,1997
2. Caractérisation agro-morphologique et moléculaire de cultivars locaux de niébé (Vigna unguiculata) du Burkina Faso;JT Ouedraogo;Camaroon Forum Biol Sci,2010
3. Allozyme diversity of cultivated cowpea Vigna unguiculata (L.) Walp;R. Pasquet;Theor Appl Genet,2000
4. Morphological and molecular diversity within Algerian cowpea (Vigna unguiculata (L.);N Ghalmi;Walp.) landraces. Genet Resour Crop Evol,2009
5. Genetic diversity and a population structure analysis of accessions in the Chinese cowpea [Vigna unguiculata (L.) Walp.] germplasm collection;H Chen;Crop J,2017
Cited by
6 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献