Abstract
Streptococcus pneumoniae (Spn) is a leading respiratory tract pathogen that colonizes the nasopharynx (NP) through adhesion to epithelial cells and immune evasion. Spn actively interacts with other microbiota in NP but the nature of these interactions are incompletely understood. Using 16S rRNA gene sequencing, we analyzed the microbiota composition in the NP of children with or without Spn colonization. 96 children were included in the study cohort. 74 NP samples were analyzed when children were 6 months old and 85 NP samples were analyzed when children were 12 months old. We found several genera that correlated negatively or positively with Spn colonization, and some of these correlations appeared to be influenced by daycare attendance or other confounding factors such as upper respiratory infection (URI) or Moraxella colonization. Among these genera, Corynebacterium showed a consistent inverse relationship with Spn colonization with little influence by daycare attendance or other factors. We isolated Corynebacterium propinquum and C. pseudodiphtheriticum and found that both inhibited the growth of Spn serotype 22F strain in vitro.
Funder
Rochester General Hospital Kidd Fund
Publisher
Public Library of Science (PLoS)
Reference61 articles.
1. Käyhty H NA, Soininen A, Väkeväinen M. The Immunological Basis for Immunization Series, Module 12: Pneumococcal Vaccines. Publications of the World Health Organization, Department of Immunization, Vaccines and Biologicals, World Health Organization. 2009.
2. Epidemiology, virulence factors and management of the pneumococcus;C Feldman;F1000Research,2016
3. Streptococcus pneumoniae: transmission, colonization and invasion;JN Weiser;Nature reviews Microbiology,2018
4. Early respiratory microbiota composition determines bacterial succession patterns and respiratory health in children;G Biesbroek;Am J Respir Crit Care Med,2014
5. Bacterial microbiota of the nasal passages across the span of human life;L Bomar;Curr Opin Microbiol,2018
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