Effects of graphene on morphology, microstructure and transcriptomic profiling of Pinus tabuliformis Carr. roots

Author:

Zhang XiaoORCID,Cao Huifen,Wang Haiyan,Zhang Runxuan,Jia Haikuan,Huang Jingting,Zhao Jianguo,Yao Jianzhong

Abstract

Graphene has shown great potential for improving growth of many plants, but its effect on woody plants remains essentially unstudied. In this work, Pinus tabuliformis Carr. bare-rooted seedlings grown outdoors in pots were irrigated with a graphene solution over a concentration range of 0–50 mg/L for six months. Graphene was found to stimulate root growth, with a maximal effect at 25 mg/L. We then investigated root microstructure and carried out transcript profiling of root materials treated with 0 and 25 mg/L graphene. Graphene treatment resulted in plasma-wall separation and destruction of membrane integrity in root cells. More than 50 thousand of differentially expressed genes (DEGs) were obtained by RNA sequencing, among which 6477 could be annotated using other plant databases. The GO enrichment analysis and KEGG pathway analysis of the annotated DEGs indicated that abiotic stress responses, which resemble salt stress, were induced by graphene treatment in roots, while responses to biotic stimuli were inhibited. Numerous metabolic processes and hormone signal transduction pathways were altered by the treatment. The growth promotion effects of graphene may be mediated by encouraging proline synthesis, and suppression of the expression of the auxin response gene SMALL AUXIN UP-REGULATED RNA 41 (SAUR41), PYL genes which encode ABA receptors, and GSK3 homologs.

Funder

Doctoral Scientific Research Foundation of Shanxi Datong University

The Program for Scientific and Technological Innovation of Higher Education Institutions in Shanxi

Science and Technology Achievements Transformation Guide project of Shanxi province

Natural Science Foundation of Shanxi Province

The National Natural Science Foundation of China

Publisher

Public Library of Science (PLoS)

Subject

Multidisciplinary

Reference65 articles.

1. Multifunctional porous graphene for nanoelectronics and hydrogen storage: New properties revealed by first principle calculations;A Du;Journal of the American Chemical Society,2010

2. Targeted graphene oxide for drug delivery as a therapeutic nanoplatform against Parkinson’s disease;S Xiong;Biomaterials Science,2021

3. Study of piezoresistive behavior of smart cement filled with graphene oxide;R Guo;Nanomaterials,2021

4. Performance evaluation of porous graphene as filter media for the removal of pharmaceutical/emerging contaminants from Water and Wastewater;A Khalil;Nanomaterials,2021

5. Acute toxicity of graphene nanoplatelets on biological wastewater treatment process;H Nguyen;Environmental Science-Nano,2016

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3