Abstract
Zika virus can infect the fetus through the placental barrier, causing ZIKV congenital syndrome and even miscarriage, which can cause great harm to pregnant women and infants. Currently, there is no vaccine and drug available to combat the Zika virus. In this study, we designed a fusion protein named EDIII-Fc, including the EDIII region of Zika E protein and human IgG Fc fragment, and obtained 293T cells that stably secreted EDIII-Fc protein using the lentiviral expression system. Mice were immunized with the EDIII-Fc protein, and it was observed that viral replication was significantly inhibited in the immunized mice compared to non-immunized mice. In rhesus macaques, we found that EDIII-Fc effectively induce the secretion of neutralizing antibodies and T cell immunity. These experimental data provide valid data for further use of Zika virus E protein to prepare an effective, safe, affordable Zika vaccine.
Funder
Science and Technology Planning Project of Guangdong Province
National Key R&D Projects of China
National Natural Science Foundation of China
Basic and Applied Basic Research Foundation of Guangdong Province
Postdoctoral Research Foundation of China
Publisher
Public Library of Science (PLoS)
Subject
Infectious Diseases,Public Health, Environmental and Occupational Health
Reference77 articles.
1. Serological and spatial analysis of alphavirus and flavivirus prevalence and risk factors in a rural community in western Kenya.;EN Grossi-Soyster;PLoS neglected tropical diseases.,2017
2. Characterization of Three New Insect-Specific Flaviviruses: Their Relationship to the Mosquito-Borne Flavivirus Pathogens;H Guzman;The American journal of tropical medicine and hygiene,2017
3. Cacipacore virus as an emergent mosquito-borne Flavivirus.;MLG Figueiredo;Revista da Sociedade Brasileira de Medicina Tropical,2017
4. A novel mechanism of antibody-mediated enhancement of flavivirus infection.;D Haslwanter;PLoS pathogens.,2017
5. Zika virus. I. Isolations and serological specificity;GW Dick;Transactions of the Royal Society of Tropical Medicine and Hygiene,1952