Abstract
Despite continued outbreaks of yellow fever virus (YFV) in endemic regions, data on its environmental stability or guidelines for its effective inactivation is limited. Here, we evaluated the susceptibility of the YFV 17D vaccine strain to inactivation by ethanol, 2-propanol, World Health Organization (WHO)-recommended hand rub formulations I and II, as well as surface disinfectants. In addition, two pathogenic strains were tested to compare inactivation kinetics by WHO-recommended hand rub formulations I and II. Furthermore, environmental stability of the vaccine strain was assessed. YFV 17D particles displayed infectivity half-life decay profiles of ~13 days at room temperature. Despite this extended environmental stability, YFV was efficiently inactivated by alcohols, WHO-recommended hand formulations, and four out of five tested surface disinfectants. These results are useful in defining disinfection protocols to prevent non-vector borne YFV transmission.
Funder
Ministry of Culture and Science of the State of North Rhine-Westphalia
Bundesministerium für Bildung und Forschung
Federal Ministry of Health
Publisher
Public Library of Science (PLoS)
Reference27 articles.
1. The global burden of yellow fever;K am Gaythorpe;Elife,2021
2. Yellow fever cases in Asia: primed for an epidemic;S Wasserman;Int J Infect Dis,2016
3. Yellow fever: an update;TP Monath;Lancet Infect Dis,2001
4. In the days of the epidemic: the 1793 yellow fever outbreak in Philadelphia as seen by physicians;J. Eckert;Trans Stud Coll Physicians Phila,1993