Abstract
Sequential dengue virus (DENV) infections often generate neutralizing antibodies against all four DENV serotypes and sometimes, Zika virus. Characterizing cross-flavivirus broadly neutralizing antibody (bnAb) responses can inform countermeasures that avoid enhancement of infection associated with non-neutralizing antibodies. Here, we used single cell transcriptomics to mine the bnAb repertoire following repeated DENV infections. We identified several new bnAbs with comparable or superior breadth and potency to known bnAbs, and with distinct recognition determinants. Unlike all known flavivirus bnAbs, which are IgG1, one newly identified cross-flavivirus bnAb (F25.S02) was derived from IgA1. Both IgG1 and IgA1 versions of F25.S02 and known bnAbs displayed neutralizing activity, but only IgG1 enhanced infection in monocytes expressing IgG and IgA Fc receptors. Moreover, IgG-mediated enhancement of infection was inhibited by IgA1 versions of bnAbs. We demonstrate a role for IgA in flavivirus infection and immunity with implications for vaccine and therapeutic strategies.
Funder
Fred Hutchinson Cancer Center Translational Data Science Integrated Research Center
NIH
Howard Hughes Medical Institute
Fred Hutchinson Cancer Center Diverse Trainee Fund
Congressionally Directed Medical Research Programs
Dr. Ralph and Marian Falk Medical Research Trust
Dr. Ralph & Marian Falk Medical Research Trust
Chan Zuckerberg Biohub - San Francisco
Fred Hutch/University of Washington/Seattle Children’s Cancer Consortium
Scientific Computing Infrastructure at Fred Hutch
Publisher
Public Library of Science (PLoS)
Subject
Virology,Genetics,Molecular Biology,Immunology,Microbiology,Parasitology
Cited by
2 articles.
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