Artificial trans-kingdom RNAi of FolRDR1 is a potential strategy to control tomato wilt disease
-
Published:2023-06-20
Issue:6
Volume:19
Page:e1011463
-
ISSN:1553-7374
-
Container-title:PLOS Pathogens
-
language:en
-
Short-container-title:PLoS Pathog
Author:
Ouyang Shou-QiangORCID,
Ji Hui-Min,
Feng Tao,
Luo Shu-Jie,
Cheng Lu,
Wang Nan
Abstract
Tomato is cultivated worldwide as a nutrient-rich vegetable crop. Tomato wilt disease caused byFusarium oxysporumf.sp.Lycopersici(Fol) is one of the most serious fungal diseases posing threats to tomato production. Recently, the development of Spray-Induced Gene Silencing (SIGS) directs a novel plant disease management by generating an efficient and environmental friendly biocontrol agent. Here, we characterized thatFolRDR1(RNA-dependent RNA polymerase 1) mediated the pathogen invasion to the host plant tomato, and played as an essential regulator in pathogen development and pathogenicity. Our fluorescence tracing data further presented that effective uptakes ofFolRDR1-dsRNAs were observed in bothFoland tomato tissues. Subsequently, exogenous application ofFolRDR1-dsRNAs on pre-Fol-infected tomato leaves resulted in significant alleviation of tomato wilt disease symptoms. Particularly,FolRDR1-RNAi was highly specific without sequence off-target in related plants. Our results of pathogen gene-targeting RNAi have provided a new strategy for tomato wilt disease management by developing an environmentally-friendly biocontrol agent.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Zhejiang Province
Publisher
Public Library of Science (PLoS)
Subject
Virology,Genetics,Molecular Biology,Immunology,Microbiology,Parasitology
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献