Abstract
Both the spindle microtubule-organizing centers and the nuclear pore complexes (NPCs) are convoluted structures where many signaling pathways converge to coordinate key events during cell division. Interestingly, despite their distinct molecular conformation and overall functions, these structures share common components and collaborate in the regulation of essential processes. We have established a new link between microtubule-organizing centers and nuclear pores in budding yeast by unveiling an interaction between the Bfa1/Bub2 complex, a mitotic exit inhibitor that localizes on the spindle pole bodies, and the Nup159 nucleoporin. Bfa1/Bub2 association with Nup159 is reduced in metaphase to not interfere with proper spindle positioning. However, their interaction is stimulated in anaphase and assists the Nup159-dependent autophagy pathway. The asymmetric localization of Bfa1/Bub2 during mitosis raises the possibility that its interaction with Nup159 could differentially promote Nup159-mediated autophagic processes, which might be relevant for the maintenance of the replicative lifespan.
Publisher
Public Library of Science (PLoS)
Subject
General Agricultural and Biological Sciences,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience
Reference74 articles.
1. Microtubule-Organizing Centers;J Wu;Annu Rev Cell Dev Biol,2017
2. A guiding torch at the poles: the multiple roles of spindle microtubule-organizing centers during cell division;AM Rincón;Cell Cycle,2020
3. Regulation of Mitotic Exit in Saccharomyces cerevisiae;B Baro;Methods Mol Biol,2017
4. Regulation of Mitotic Exit by Cell Cycle Checkpoints: Lessons From Saccharomyces cerevisiae;L Matellan;Genes (Basel),2020
5. Inhibition of the mitotic exit network in response to damaged telomeres;M Valerio-Santiago;PLoS Genet,2013