Genomic insights into host and parasite interactions during intracellular infection by Toxoplasma gondii

Author:

Ulahannan Netha,Cutler RonaldORCID,Doña-Termine Reanna,Simões-Pires Claudia A.,Wijetunga N. AriORCID,Croken Matthew McKnightORCID,Johnston Andrew D.ORCID,Kong Yu,Maqbool Shahina B.,Suzuki MasakoORCID,Greally John M.ORCID

Abstract

To gain insights into the molecular interactions of an intracellular pathogen and its host cell, we studied the gene expression and chromatin states of human fibroblasts infected with the Apicomplexan parasiteToxoplasma gondii. We show a striking activation of host cell genes that regulate a number of cellular processes, some of which are protective of the host cell, others likely to be advantageous to the pathogen. The simultaneous capture of host and parasite genomic information allowed us to gain insights into the regulation of theT.gondiigenome. We show how chromatin accessibility and transcriptional profiling together permit novel annotation of the parasite’s genome, including more accurate mapping of known genes and the identification of new genes andcis-regulatory elements. Motif analysis reveals not only the knownT.gondiiAP2 transcription factor-binding site but also a previously-undiscovered candidate TATA box-containing motif at one-quarter of promoters. By inferring the transcription factor and upstream cell signaling responses involved in the host cell, we can use genomic information to gain insights intoT.gondii’sperturbation of host cell physiology. Our resulting model builds on previously-described human host cell signalling responses toT.gondiiinfection, linked to induction of specific transcription factors, some of which appear to be solely protective of the host cell, others of which appear to be co-opted by the pathogen to enhance its own survival.

Funder

National Institute of Neurological Disorders and Stroke

National Institute of General Medical Sciences

National Institute on Aging

Publisher

Public Library of Science (PLoS)

Subject

Multidisciplinary

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3