Multiplex short tandem repeat profiling of immortalized hepatic stellate cell line Col-GFP HSC

Author:

Meurer Steffen K.,Brenner David A.,Weiskirchen RalfORCID

Abstract

Misidentification, cross-contamination and genetic drift of continuous animal cell lines are persistent problems in biomedical research, leading to erroneous results and inconsistent or invalidated studies. The establishment of immortalized hepatic stellate cell line Col-GFP HSC was reported in PLoS One in the year 2013. In the present study a multi loci short tandem repeat signature for this cell line was established that allows for unique cell line authentication.

Funder

Deutsche Forschungsgemeinschaft

Publisher

Public Library of Science (PLoS)

Subject

Multidisciplinary

Reference19 articles.

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4. Incorrect strain information for mouse cell lines: sequential influence of misidentification on sublines;K Uchio-Yamada;In Vitro Cell Dev Biol Anim,2017

5. Cell line misidentification: the beginning of the end;American Type Culture Collection Standards Development Organization Workgroup ASN-0002;Nat Rev Cancer,2010

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