Abstract
Background
Although some human studies have reported gut microbiome changes in individuals with Alzheimer’s disease (AD) dementia or mild cognitive impairment (MCI), gut microbiome alterations in preclinical AD, i.e., cerebral amyloidosis without cognitive impairment, is largely unknown.
Objective
We aimed to identify gut microbial alterations associated with preclinical AD by comparing cognitively normal (CN) older adults with cerebral Aβ deposition (Aβ+ CN) and those without cerebral Aβ deposition (Aβ− CN).
Methods
Seventy-eight CN older participants (18 Aβ+ CN and 60 Aβ− CN) were included, and all participants underwent clinical assessment and Pittsburg compound B–positron emission tomography. The V3–V4 region of the 16S rRNA gene of genomic DNA extracted from feces was amplified and sequenced to establish the microbial community.
Results
Generalized linear model analysis revealed that the genera Megamonas (B = 3.399, q<0.001), Serratia (B = 3.044, q = 0.005), Leptotrichia (B = 5.862, q = 0.024) and Clostridium (family Clostridiaceae) (B = 0.788, q = 0.034) were more abundant in the Aβ+ CN group than the Aβ− CN group. In contrast, genera CF231 (B = −3.237, q< 0.001), Victivallis (B = −3.447, q = 0.004) Enterococcus (B = −2.044, q = 0.042), Mitsuokella (B = −2.119, q = 0.042) and Clostridium (family Erysipelotrichaceae) (B = −2.222, q = 0.043) were decreased in Aβ+ CN compared to Aβ− CN. Notably, the classification model including the differently abundant genera could effectively distinguish Aβ+ CN from Aβ− CN (AUC = 0.823).
Conclusion
Our findings suggest that specific alterations of gut bacterial taxa are related to preclinical AD, which means these changes may precede cognitive decline. Therefore, examining changes in the microbiome may be helpful in preclinical AD screening.
Funder
Ministry of Science and ICT
Ministry of Health and Welfare
Seoul National University Hospital
National Institute on Aging
Publisher
Public Library of Science (PLoS)
Cited by
12 articles.
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