Abstract
The mosquito Aedes spp. holds important relevance for human and animal health, as it serves as a vector for transmitting multiple diseases, including dengue and Zika virus. The microbiome’s impact on its host’s health and fitness is well known. However, most studies on mosquito microbiomes have been conducted in laboratory settings. We explored the mixed microbial communities within Aedes spp., utilizing the 16S rRNA gene for diversity analysis and shotgun metagenomics for functional genomics. Our samples, which included Ae. aegypti and Ae. albopictus, spanned various developmental stages—eggs, larvae, and adults—gathered from five semiurban areas in Mexico. Our findings revealed a substantial diversity of 8,346 operational taxonomic units (OTUs), representing 967 bacterial genera and 126,366 annotated proteins. The host developmental stage was identified as the primary factor associated with variations in the microbiome composition. Subsequently, we searched for genes and species involved in mosquito biocontrol. Wolbachia accounted for 9.6% of the 16S gene sequences. We observed a high diversity (203 OTUs) of Wolbachia strains commonly associated with mosquitoes, such as wAlb, with a noticeable increase in abundance during the adult stages. Notably, we detected the presence of the cifA and cifB genes, which are associated with Wolbachia’s cytoplasmic incompatibility, a biocontrol mechanism. Additionally, we identified 221 OTUs related to Bacillus, including strains linked to B. thuringiensis. Furthermore, we discovered multiple genes encoding insecticidal toxins, such as Cry, Mcf, Vip, and Vpp. Overall, our study contributes to the understanding of mosquito microbiome biodiversity and metabolic capabilities, which are essential for developing effective biocontrol strategies against this disease vector.
Funder
Universidad Autónoma Metropolitana
CONACyT
Publisher
Public Library of Science (PLoS)
Reference88 articles.
1. Feeding Behavior, Natural Food, And Nutritional Relationships Of Larval Mosquitos;R. Merritt;Annual Review of Entomology,1992
2. Multimodal floral cues guide mosquitoes to tansy inflorescences;H Peach D A;Scientific Reports,2019
3. Mosquitoes host communities of bacteria that are essential for development but vary greatly between local habitats;L Coon K;Molecular Ecology,2016
4. Carryover effects of larval exposure to different environmental bacteria drive adult trait variation in a mosquito vector;B Dickson L;Science Advances,2017
5. Dynamic Gut Microbiome across Life History of the Malaria Mosquito Anopheles gambiae in Kenya;Y Wang;PLoS ONE,2011
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献