Abstract
To analyze the results of proficiency testing for anti-tuberculosis drug susceptibility testing (DST) in China. Number of laboratory participating the proficiency testing performed DST, and the sensitivity, specificity, reproducibility, and accordance rate were calculated from data of 13 rounds proficiency testing results for DST from 2008 to 2021. A total of 30 and 20 strains of Mycobacterium tuberculosis with known susceptibility results were sent to each laboratory in 2008 to 2019, 2020 and 2021, respectively. The number of participating laboratories ranged from 30 in 2009 to 546 in 2021. L-J DST was the predominant method. The specificity presented relatively higher than sensitivity. Improvement of specificity were observed for all drugs through the years, while sensitivity did not show improvement for amikacin and capreomycin. Accordance rate of pyrazinamide and kanamycin and reproducibility of capreomycin and pyrazinamide were not significantly improved through the years. Most of the participating laboratories significantly improved the quality of their DST through the consecutive rounds of proficiency testing except for second-line injectable drugs and pyrazinamide. The results highlight the importance of developing novel and/or improving existing methods for phenotypic DST for certain drugs.
Funder
Central Government Fund for Tuberculosis Control and Prevention Project
Publisher
Public Library of Science (PLoS)
Reference24 articles.
1. World Health Organization. Global Tuberculosis Report 2022. Geneva: WHO; 2022. https://www.who.int/publications/i/item/9789240061729
2. Members of National and Regional Tuberculosis Reference Laboratories. Proficiency analysis of drug susceptibility testing by national-level tuberculosis reference laboratories from 1995 to 2003;G-H Bai;J Clin Microbiol,2007
3. Drug susceptibility testing proficiency in the network of supranational tuberculosis reference laboratories;A Van Deun;Int J Tuberc Lung Dis,2011
4. Evaluation standard of external quality assessment programme for drug susceptibility testing of mycobacterium tuberculosis in Japanese laboratories: proficiency testing in 2004–2010;The Committee for Mycobacterial Examinations;Kekkaku,2015
5. Proficiency of drug susceptibility testing for Mycobacterium tuberculosis in Taiwan, 2007–2011;M-H Wu;Int J Tuberc Lung Dis,2013