Disentangling primer interactions improves SARS-CoV-2 genome sequencing by multiplex tiling PCR

Author:

Itokawa KentaroORCID,Sekizuka TsuyoshiORCID,Hashino Masanori,Tanaka Rina,Kuroda MakotoORCID

Funder

Japan Agency for Medical Research and Development

Publisher

Public Library of Science (PLoS)

Subject

Multidisciplinary

Reference23 articles.

1. Towards a genomics-informed, real-time, global pathogen surveillance system;JL Gardy;Nat Rev Genet,2017

2. NextStrain: Real-time tracking of pathogen evolution;J Hadfield;Bioinformatics,2018

3. A novel coronavirus from patients with pneumonia in China;N Zhu;N Engl J Med,2019

4. An amplicon-based sequencing framework for accurately measuring intrahost virus diversity using PrimalSeq and iVar;ND Grubaugh;Genome Biol,2019

5. Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples.;J Quick;Nat Protoc.,2017

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