Abstract
As a strategy for minimizing microbial infections in fish hatcheries, we have investigated how putatively probiotic bacterial populations influence biofilm formation. All surfaces that are exposed to the aquatic milieu develop a microbial community through the selective assembly of microbial populations into a surface-adhering biofilm. In the investigations reported herein, we describe laboratory experiments designed to determine how initial colonization of a surface by nonpathogenic isolates from sturgeon eggs influence the subsequent assembly of populations from a pelagic river community, into the existing biofilm. All eight of the tested strains altered the assembly of river biofilm in a strain-specific manner. Previously formed isolate biofilm was challenged with natural river populations and after 24 hours, two strains and two-isolate combinations proved highly resistant to invasion, comprising at least 80% of the biofilm community, four isolates were intermediate in resistance, accounting for at least 45% of the biofilm community and two isolates were reduced to 4% of the biofilm community. Founding biofilms of Serratia sp, and combinations of Brevundimonas sp.-Hydrogenophaga sp. and Brevundimonas sp.-Acidovorax sp. specifically blocked populations of Aeromonas and Flavobacterium, potential fish pathogens, from colonizing the biofilm. In addition, all isolate biofilms were effective at blocking invading populations of Arcobacter. Several strains, notably Deinococcus sp., recruited specific low-abundance river populations into the top 25 most abundant populations within biofilm. The experiments suggest that relatively simple measures can be used to control the assembly of biofilm on the eggs surface and perhaps offer protection from pathogens. In addition, the methodology provides a relatively rapid way to detect potentially strong ecological interactions between bacterial populations in the formation of biofilms.
Funder
Michigan Department of Natural Resources State Wildlife Grants Program
Michigan State University Water Cube Initiative
Michigan State University College of Natural Science Dissertation Completion Award
Publisher
Public Library of Science (PLoS)
Reference115 articles.
1. Industrial arsenic contamination causes catastrophic changes in freshwater ecosystems.;G Chen;Scientific reports.,2015
2. Beyond the ocean: contamination of freshwater ecosystems with (micro-) plastic particles.;R Dris;Environmental chemistry,2015
3. Effects of organic herbicides on phototrophic microbial communities in freshwater ecosystems;S Pesce;Reviews of environmental contamination and toxicology,2012
4. Micro-(nano) plastics in freshwater ecosystems: abundance, toxicological impact and quantification methodology.;S-A Strungaru;TrAC trends in analytical chemistry,2019
5. Herbicide contamination of freshwater ecosystems: impact on microbial communities.;A Villeneuve;Pesticides-Formulations, effects, fate.,2011