DETERMINATION OF THE QUANTITATIVE CONTENT OF PROSTAGLANDIN F2ALPHA AS A MENSTRUCTURAL MARKER

Abstract

Relevance. The issue of determining the origin of blood on material evidence is of fundamental importance to criminal investigations. But today in forensic practice there is no reliable method for determining the blood of menstrual origin. Therefore, it is important to look for a criterion that would be a reliable marker of menstrual blood. Objective: To investigate the possibility of using prostaglandin F2alpha (PGF2α) as a marker of menstrual blood origin. Materials and methods. Practically healthy women (n = 51) of reproductive age were examined. In group 1 (n = 28) were women 18-29 years, in group 2 (n = 23) - 30-45 years. During the examination in the women's consultation, a sample of vaginal contents during menstrual period on a tampon, a sample of vaginal contents in the extra-menstrual period and a sample of capillary blood on gauze were removed. The removed objects were dried. Prostaglandins in the samples were determined by the method of preparative isolation and systematic analysis of prostaglandins obtained by the method of biosynthesis. The quantitative content of PGF2α in the blood was evaluated directly on chromatograms, taking into account the relationship between spot area and object mass. The resulting digital material was processed statistically by variational statistics methods, with mean, deviation, mean error, Student's t-test of significant differences. The contents of PG F2α in different liquids were compared. The differences between the liquids were considered significant under the condition P <0.001. Results. There is a dependence of PGF2α content on women's age and on the regional origin of the fluid. In women 30-45 years, the content of PGF2α is always higher in all fluids than in women 18-29 years (p <0.001): in the vaginal fluid - by 11%; in menstrual blood - by 6%; in capillary blood - by 3%.The content of PGF2α is highest in menstrual blood, compared to vaginal fluid and capillary blood. This is true for women of both ages: in women 30-45 years PGF2α content is 2.6 times higher in menstrual blood than in capillary blood, and 1.4 times higher than in vaginal fluid; in women 18-29 years PGF2α content is 2.7 times higher in menstrual blood than in capillary blood, and 1.5 times higher than in vaginal fluid. Conclusion. A content of PGF2α above 13.1 ng / mg of dry blood tissue is a sure sign of menstrual blood origin.