IMPROVEMENT OF THE ANALYTICAL CONTROL FOR APPLICATION OF PESTICIDES IN THE SYSTEM OF CHEMICAL PROTECTION OF CHICKPEA

Abstract

Relevance. To date, there are no approved methods in Ukraine for the determination of azoxystrobin, thiabendazole, fludioxonil and pyridate in grain chickpeas. Therefore, there is a need for scientific substantiation of the method selection, the development of conditions for sample preparation of chickpea grain, qualitative identification and quantification of pesticides in this matrix used in protection system of chickpea was given in the article, which will allow to control the established hygienic standards of pesticides and minimize pesticide negative impact on population health and the environment. Objective of the research is to develop methods for the determination of azoxystrobin, thiabendazole, fludioxonil and pyridate in grain chickpea. Materials and methods. Chromatographic analysis was performed by Shimazu (Japan) liquid chromatographs using ultraviolet and fluorescent detection. The package of IBM SPSS StatisticsBase v.22 and MS Exel statistical programs was used for statistical processing of results. Results. Optimal conditions of sample preparation of chickpea grain and chromatographic determination of azoxystrobin, fludioxonil, thiabendazole and pyridate (as the sum of pyridate and its metabolite – pyridafol) with the limits of quantitative determination of 0.1; 0.1; 0.01 and 0.05 mg/kg, respectively, wеre developed to provide the determination of the analyzed compounds at the required level (the measure of correctness is (72–75)%) and the control of the established hygienic standards of these compounds in chickpea grain. Conclusions. Developed methods for determination of azoxystrobin, fludioxonil, thiabendazole and pyridate in chickpea grain by high-performance liquid chromatography allow to control the established hygienic standards, to obtain representative information on the content of pesticide residues, which is a prerequisite for risk assessment of plant protection products.