DETERMINATION OF THE ACTUAL PROPERTIES OF GLUCOSAMIN HYDROCHLORIDE IN EXPERIMENTS WITH SUFFICIENTLY SPEED PHYSICAL LOADING

Abstract

Relevance. Glucosamine hydrochloride, as a medicament, is introduced in various fields of medicine. Its frigidoid and cardioprotective properties have been determined, but its protective properties have not been determined yet. There is a need to substantiate the expediency of using glucosamine hydrochloride as a metabolic and metabolitotropic agent, as improving performance and accelerating recovery processes under extreme conditions and under physical stress. Objective. Investigate possible acto-protective properties of glucosamine hydrochloride in an experiment on rats at high-speed physical activity. Materials and methods. Experiments were performed on 35 white rats in males of the Wistar line, weighing 180-200 g. Rapid loading was created by running rats at Tredbani with a tape speed of 42 m / min and on the test "swimming with additional load to full exhaustion". Glucosamine hydrochloride was administered intragastrically at a dose of 50 mg / kg using a probe for 10 days. Under the thiopental-sodium anesthesia, decapitations were performed, myocardial, liver, and skeletal muscle were removed, in which the content of the components of prooxidant-antioxidant homeostasis (level of LO-diene conjugates (DK), secondary malonic dialdehydes (MDA), superoxide dismutase enzymes activity (SOD), catalase). Results. Introduction of glucosamine hydrochloride lengthened swimming time in rats by 59% and running time by 52%, which indicates the adaptive and act-protective effect of the agent. During a high-speed run, without the use of an actoprotector, the level of lipid peroxidation (DC, MDA) increases and the activity of antioxidant protection enzymes (SOD, catalase) decreases. However, when using glucosamine hydrochloride, the level of lipid peroxidation (DC, MDA) decreases and the level of antioxidant protection (SOD, catalase) increases, which proves its act-protective properties. So, glucosamine hydrochloride prevents a change in the indices of prooxidant-antioxidant homeostasis, which is explained by the presence of a membranotropic and antioxidant effect in it. Conclusions. Glucosamine hydrochloride has its protective properties. The implementation of the actoprotective action may be mediated due to antioxidant effects.