Corrections for matrix effects on fluorescence measurement of a multi-platform optical sensor-Reference-Cited by-同舟云学术

Corrections for matrix effects on fluorescence measurement of a multi-platform optical sensor

Published:2016-09-01 Issue:3 Volume:11 Page:644-660
ISSN:1751-231X
Container-title:Water Practice and Technology
language:en
Short-container-title:

Author:

Ng Chee-Loon¹,Ng Yan-Jie¹,Chen Qing-Qing¹,Hemond Harold F.²

Affiliation:

1. Singapore-MIT Alliance for Research and Technology (SMART) Centre, 1 Create way, CREATE Tower, #09-03, 138602, Singapore, Singapore

2. Massachusetts Institute of Technology, Cambridge, Building 48, 15 Vassar Street, Cambridge, MA 02139, USA

Abstract

The LEDIF (LED-induced fluorescence) is an in situ optical instrument that utilizes fluorescence, absorbance, and scattering to identify and quantify substances in water bodies. In this study, matrix effects on fluorescence signals caused by inner filtering, temperature, intramolecular deactivation, turbidity, and pH were investigated, and compensation equations developed to correct measured values and improve accuracy. Multiple simultaneous matrix effect corrections were demonstrated with a laboratory sample subjected to known interferences and physical conditions. In general, compensation was found to be important to improve the accuracy of fluorescence measurements.

Publisher

IWA Publishing

Subject

Water Science and Technology

Link

http://iwaponline.com/wpt/article-pdf/11/3/644/381048/wpt0110644.pdf

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