Recovery of Enterovirus from Primary Sludge Using Three Elution Concentration Procedures

Abstract

Recovery of virus from sewage sludge is usually performed using an elution of adsorbed virus with alkaline pH solution followed by a concentration step before inoculation on cell cultures. In the present study three extraction-concentration procedures have been compared testing 40 g of solid fraction sludge. The highest virus recovery is obtained using an elution with 3 % beef extract - 0.1 M borate buffer pH 9 associated to a polyethyleneglycol precipitation as concentration step. Heterogeneous and lower results are obtained when the elution step uses a 1 % skim milk solution pH 9 or a glycin 0.05 M solution pH 9 and an organic flocculation as concentration step.