The use of enzyme-linked immunosorbent assays (ELISA) for the determination of pollutants in environmental and industrial wastes

Author:

Hirobe M.1,Goda Y.1,Okayasu Y.2,Tomita J.3,Takigami H.4,Ike M.5,Tanaka H.6

Affiliation:

1. Japan EnviroChemicals, Ltd., 2-17-85 Juso-Honmachi Yodogawa-ku, Osaka, 532-0024, Japan (E-mail: hirobe_masato@jechem.co.jp; goda_yasuhiro@jechem.co.jp)

2. Public Works Research Institute, 1-6 Minamihara, Tsukuba, 305-8516, Japan (E-mail: okayasu@pwri.go.jp)

3. Tokyo Electric Power Environmental Engineering Co., Inc., 2-3-6 Ohnodai midori-ku, Chiba, 267-0056, Japan (E-mail: tomita-jun@mail.tee-kk.co.jp)

4. National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Japan (E-mail: takigami@nies.go.jp)

5. Graduate School of Engineering, Osaka University, 2-1 Yamada-oka Suita, Osaka, 565-0872, Japan (E-mail: ike@env.eng.osaka-u.ac.jp)

6. Graduate School of Engineering, Kyoto University, 1-2 Yumihama, Otsu, Shiga 520-0811, Japan (E-mail: htanaka@biwa.egc.kyoto-u.ac.jp)

Abstract

Twelve enzyme-linked immunosorbent assays (ELISA), for the determination of surfactants [linear alkylbenzene sulfonates (LAS), alkyl ethoxylates (AE), and alkylphenol ethoxylates (APE)], endocrine disruptors [alkylphenol (AP), AP + APE, and bisphenol A (BPA)], estrogens [17beta-estradiol (E2), estrone (E1), estrogen (ES: E1 + E2 + estriol (E3)), 17alfa-ethynylestradiol (EE2)], dioxins and polychlorinated biphenyls (PCBs), were validated on environmental water and industrial wastes. The lowest quantification limits of these ELISAs were 0.05 μg/L (BPA, E2, E1, ES and EE2), 2 μg/L (AE), 3 μg/L (dioxins and PCBs), 5 μg/L (AP, AP + APE) and 20 μg/L (LAS and APE). To apply these ELISAs to environmental or industrial waste samples, simple and appropriate pre-treatment methods were also developed for each ELISA. With optimized pre-treatments, the values of ELISAs were well co-related, in all cases, to those of instrumental analytical methods such as liquid chromatography (HPLC), liquid chromatography-tandem mass spectrometry (LC-MS/MS), and high-resolution gas chromatography mass spectrometry (HR-GC-MS), etc.

Publisher

IWA Publishing

Subject

Water Science and Technology,Environmental Engineering

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