High nitrite concentration accelerates nitrite oxidising organism's death

Abstract

Abstract High nitrite is a known operation parameter to inhibit the biological oxidation of nitrite to nitrate. The phenomenon is traditionally expressed using a Monod-type equation with non-competitive inhibition, in which the reaction associated with the biomass growth is reduced when high nitrite is present. On the other hand, very high nitrite is also known to slay nitrifiers. To clarify the difference between the growth inhibition and the poisoning, cell counting for living microorganisms in the nitrite oxidiser-enriched activated sludge was conducted in batch conditions under various nitrite concentrations together with measurements of biomass chemical oxygen demand (COD) concentration and oxygen uptake rate. The experiments demonstrated that these measureable parameters were all decayed when nitrite concentration exceeded 100–500 mgN/L at pH 7.0 in the system, indicating that nitrite poisoning took place. Biomass growth was recognised in lower range of nitrite which was expressed with growth inhibition only. Based on the response, a kinetic model for the biological nitrite oxidation was developed with a modification of IWA ASM1. The model was further utilised to calculate a possibility to wash out nitrite oxidiser in the aeration tank where a part of the return activated sludge was exposed to high nitrite liquor in a side-stream partial nitritation reactor.