Analysis of energy recovery and microbial community in an amalgamated CSTR-UASBs reactor for a three-stage anaerobic fermentation process of cornstalks

Author:

Zhao Bowei1,Xie Fei2,Zhou Aijuan1,Liu Zhihong1,Ji Li2,Zhang Guixiang2,Yue Xiuping1

Affiliation:

1. a College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China

2. b School of Environmental Science and Engineering, Taiyuan University of Science and Technology, Taiyuan 030024, China

Abstract

Abstract In this study, a continuous stirred-tank reactor (CSTR) coupled with up-flow anaerobic sludge beds (UASBs) reactor was successfully developed for enhancing methane production and carbon recovery rate from cornstalks. Acetic acid production was higher in regions A than in B and C. The methane percentage achieved at 75.98% of total gas and methane production of cornstalks was up to 520.07 mL/g, during the stable operation period. The carbon of recovery rate, represented substrates converted to methane gas, reached 69.32% in stable stage. Microbial community structure analysis revealed that Paludibacter, Prevotella/Clostridium sensu stricto, and Caldisericum were the dominant bacteria for the degradation of cellulose, lignin, and other refractory macromolecules in regions A, B, and C, respectively. Methanobacterium and Methanolinea were the two major genera, accounting for methanogenesis generation.

Funder

the Ministry of Environmental Protection of the People's Republic of China

the National Natural Science Foundation of China

National Natural Science Foundation of China

Yalong River Joint Fund

the Foundation Research Program of Shanxi Province

Publisher

IWA Publishing

Subject

Water Science and Technology,Environmental Engineering

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