Affiliation:
1. Department of Environmental Science and Engineering, Kwangju Institute of Science and Technology(K-JIST) and the Advanced Environmental Monitoring Research Center(ADEMRC), 1 Oryong-dong, Puk-gu, Kwangju 506-712, Korea
Abstract
With respect to developing a more sensitive biosensor, a recombinant fluorescent Chinese Hamster Ovary cell line was used for the monitoring of various toxicants. Both cell lines, EFC-500 and KFC-A10, were able to detect toxicants sensitively. They were characterized with mitomycin C and γ-ray as genotoxicants and bisphenol A, nonylphenol, ziram and methyl bromide as possible and known EDCs. When compared to each other, the response of KFC-A10 was generally more informative and sensitive. Compared to typical bacterial biosensor systems, these cell lines offered a sensitivity of 2- to 50-fold greater for the tested chemicals. Based on these results, the use of mammalian cells offers a sensitive biosensor system that is not only fast, cheap and reproducible but also capable of monitoring the endocrine-like characteristics of environmental toxicants.
Subject
Water Science and Technology,Environmental Engineering
Cited by
14 articles.
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