Characterization of type 1851 organism isolated from activated sludge samples

Abstract

Five orange pigmented filamentous strains of Eikelboom's type 1851 were isolated from bulking activated sludge, and were examined for their phylogenetic lineage, morphology, and physiology. The analysis of 16S rDNA sequence revealed that the isolates belong to Chloroflexus subdivision in green non sulfur bacteria. They shared most common features with type 1851, but the result of Gram stain was negative. Furthermore, they showed polymorphic nature and gliding motility, which could not be seen in activated sludge samples. General physiological tests were conducted under aerobic conditions although they could also grow by fermentation. Good growth was generally seen on sugars. The extremely slow growth rate (0.48–0.93 day−1) suggests the stimulation of this type exclusively in long SRT systems fed with sugars.