Affiliation:
1. 1 Nitte (Deemed to be University), Nitte University Centre for Science Education and Research, Mangaluru, India
Abstract
Abstract
The present study investigated the predatory efficacy of Bdellovibrio isolated from untreated hospital wastewater sources against human pathogens. Bdellovibrio is a Gram-negative, motile, uniflagellate predatory bacteria present in the environment, which directly predates on other bacteria, including human pathogens. In the present study, 30 hospital effluent samples were collected and screened for Bdellovibrio. A total of 11 Bdellovibrio isolates were obtained by the double-layer agar technique. All the isolates were identified by amplification of the 16S rDNA coding region using polymerase chain reaction (PCR) and confirmed as Bdellovibrio stolpii. The lytic activity of confirmed isolates was investigated against four Gram-negative bacteria Pseudomonas aeruginosa ADW44, Pseudomonas aeruginosa 27853(ATCC Strain), V. cholerae and Salmonella typhimurium of clinical origin obtained from the institutional repository. Among the 11 isolates, three Bdellovibrio isolates NBd1, YBd1 and RBd1 demonstrated the ability to prey upon the tested clinical isolates. To the best of our knowledge, this is the first report on the isolation of B. stolpii from hospital wastewater settings in India with broad and high bacteriolytic activity.
Subject
Infectious Diseases,Microbiology (medical),Public Health, Environmental and Occupational Health,Waste Management and Disposal,Water Science and Technology
Reference29 articles.
1. Predatory bacteria as living antibiotics–where are we now?;Microbiology,2021
2. Antibiotic susceptibility testing by a standardized single disk method;American Journal of Clinical Pathology,1966
3. Insight into the possible use of the predator Bdellovibrio bacteriovorus as a probiotic;Nutrients,2020
4. Biotechnological potential of Bdellovibrio and like organisms and their secreted enzymes;Frontiers in Microbiology,2020
5. Bacterial invasion and killing by predatory Bdellovibrio primed by predator prey cell recognition and self-protection;Current Opinion in Microbiology,2020
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献