Identification of antibiotic-resistant, gram-negative bacteria in sewage and bioaerosols from a wastewater treatment plant: A genotypic and phenotypic study-Reference-Cited by-同舟云学术

Identification of antibiotic-resistant, gram-negative bacteria in sewage and bioaerosols from a wastewater treatment plant: A genotypic and phenotypic study

Published:2024-06-04 Issue:7 Volume:22 Page:1153-1161
ISSN:1477-8920
Container-title:Journal of Water and Health
language:en
Short-container-title:

Author:

da Silva Luiz Vitor¹^ORCID,Medeiros Sandra Helena Westrupp²,Schneider Andréa Lima Dos Santos²^ORCID,dos Santos-Silva Jéssica Caroline³^ORCID,Fachini Souza André Luis⁴^ORCID,Rhoden Sandro Augusto⁵

Affiliation:

1. a Master in Environmental Sciences – Instituto Federal Catarinense – IFC, Araquari, SC, Brazil

2. b PhD in Chemical Engineering – Professor at the University of Joinville Region – UNIVILLE, Joinville, SC, Brazil

3. c PhD in Water Resources and Environmental Engineering – Federal University of Paraná – UFPR Curitiba, Curitiba PR, Brazil

4. d PhD in Biochemical Sciences – Professor at the Federal Institute of Santa Catarina – IFC Araquari, Araquari SC, Brazil

5. e PhD in Comparative Biology – Professor at the Federal Institute of Santa Catarina – IFC São Francisco do Sul, Florianópolis SC, Brazil

Abstract

ABSTRACT Antimicrobial resistance (AMR) is a growing global public health concern. This study aimed to investigate the presence of bacteria and antibiotic resistance genes in the environment, more specifically in sewage and bioaerosol samples collected at a Wastewater Treatment Plant (WWTP). Bacterial species were identified and tested for antibiotic sensitivity. In addition, bla CTX-M, bla SHV, bla TEM and bla KPC genotypes, including those related to resistance to carbapenems, were detected by PCR. The results of this research are extremely important for understanding the mechanisms involved in antimicrobial resistance and for preventing the dissemination of these antibiotic-resistant microorganisms. This study contributes to the identification of sources of antibiotic resistance in the environment and implementation of AMR control and prevention strategies while preserving the effectiveness of antibiotics and public health.

Publisher

IWA Publishing

Link

https://iwaponline.com/jwh/article-pdf/22/7/1153/1454558/jwh0221153.pdf

Reference36 articles.

1. Mechanisms of Action of Carbapenem Resistance

2. CTX-M Enzymes: Origin and Diffusion

3. CETESB 2011 Companhia Ambiental do Estado de São Paulo, Guia Nacional de Coleta e Preservação de Amostras: Água, Sedimento, Comunidades Aquáticas e Efluentes Líquidos. São Paulo: CETESB; Brasília: ANA.

4. European Committee on Antimicrobial Susceptibility Testing (EUCAST) 2022 Breakpoint tables for interpretation of MICs and zone diameters. Version 12.0 2022. Available from: https://www.eucast.org/clinical_breakpoints/ (accessed 12 June 2022).

5. Solar-light driven photodegradation of antimicrobials, their transformation by-products and antibiotic resistance determinants in treated wastewater