Affiliation:
1. Department of Medical Technology, Faculty of Pharmacy, University of Santo Tomas, 1008 Manila, Philippines
Abstract
ABSTRACT
Acanthamoebae spp. are considered the most commonly occurring free-living amoebae (FLA) in the environment. Their high resilience enables them to thrive in different types of environments. Using purposive sampling, 80 surface water samples were collected from identified coastal sites in Mariveles, Bataan, and Lingayen Gulf (40 water samples for each). Nineteen (23.75%) of the 80 water samples yielded positive amoebic growth during the 14-day culture and microscopic examination. The polymerase chain reaction confirmed Acanthamoeba spp. DNA in isolates MB1, A3, A4, A7, C5, and D3 using JDP1 and JDP2 primer sets. Further sequencing revealed that the isolates belonged to Acanthamoeba sp., Acanthamoeba culbertsoni, Acanthamoeba castellani, and Acanthamoeba genotype T4. The sequences were deposited in GenBank and registered under accession numbers PP741651, PP767364, PP741728, PP741729, PP767365, and PP767366, respectively. Potential risk factors such as waste disposal, expansion of human settlements to coastal locations, and soil runoffs in these environments should be controlled to mitigate the proliferation of potentially pathogenic strains of FLAs.
Reference32 articles.
1. Isolation and molecular identification of Acanthamoeba spp. from hospital dust and soil of Khomein, Iran, as reservoir for nosocomial infection;Parasite Epidemiology and Control,2021
2. Identification and distribution of Acanthamoeba species genotypes associated with nonkeratitis infections;The Journal of Clinical Microbiology,2005
3. Acanthamoeba keratitis: Confirmation of the UK outbreak and a prospective case-control study identifying contributing risk factors;The British Journal of Ophthalmology,2018
4. First report of Acanthamoebae spp. isolation from a volcanic mud spring in the Philippines;Journal of Water & Health,2023
5. Centers for Disease Control and Prevention (CDC)
2019
Free Living Amebic Infections. DPDx-Laboratory Identification of Parasites of Public Health Concern. Global Health, Division of Parasitic Diseases and Malaria. Available from: https://www.cdc.gov/dpdx/freeLivingAmebic/index.html (accessed 25 February 2024).