Inactivation of Ascaris for thermal treatment and drying applications in faecal sludge

Abstract

Abstract Ascaris lumbricoides is the most common helminth of human health importance, and the most resilient helminth found in faecal sludge. There are numerous types of sludge treatments; however, heating and drying are most commonly used for pathogen inactivation. Ascaris suum eggs were heated in a water bath at 40–55 °C for 10 seconds to 60 minutes in water, as well as heated in both urine diversion dry toilet and ventilated improved pit latrine sludge at 40 °C, 60 °C and 80 °C for times ranging from 5 seconds to 120 minutes. Eggs were also spiked into sludges of different moisture contents and incubated over 12 weeks at 25 °C, with samples analysed weekly. Overall, we concluded that eggs were inactivated at temperatures >50 °C, that the temperature–time relationship directly impacted the efficacy of heat treatment, that suspension medium had no effect, and that eggs survived better in wet rather than dry sludges.