Abstract
The surface imprinted technique was utilized in creating the synthetic lysozyme receptors on the poly(hydroxyethyl methacrylate) [p(HEMA)] cryogel membrane with the assistance of polydopamine under alkaline conditions. Following the design of the artificial receptors, Fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and the swelling test were used for the characterization studies and the lysozyme adsorption capability of the adsorbent was evaluated in the aqueous solution.
The maximum adsorption capacity (Qmax) of lysozyme was found as 108.9 mg/g and the adsorption of the target protein on the adsorbent was monolayer and showed a good fit to the Langmuir isotherm model. The IF factor was 3.26 and the adsorbent was highly selective against creatinine and bovine serum albumin (BSA). Furthermore, the adsorbent maintained its stability after 5 adsorption, desorption, and regeneration cycles.
Reference32 articles.
1. Topcu, AA, Kılıc, S, Özgür, E, Türkmen, D, Denizli, A. Inspirations of biomimetic affinity ligands: a review. ACS Omega 7 (2022) 32897-32907.
2. Bereli et al., Molecular imprinting technology for biomimetic assemblies. Hacettepe J. Biol. & Chem., 48 (5) (2020) 575-601.
3. Haupt, K Peer reviewed: molecularly imprinted polymers: the next generation. Anal. Chem, 75 (17) (2003) 376 A-383 A.
4. Dong, C, Shi, H, Han, Y, Yang, Y, Wang, R, Men, J. Molecularly imprinted polymers by the surface imprinting technique. Eur. Polym. J, 145 (2021) 110231.
5. Dena, ASA, Ali, AM, El-Sherbiny IM. Surface-imprinted polymers (SIPs): Advanced Materials for bio-recognition. J. Nanotech. & Adv. Mat, 8 (1) (2020) 1-19.