Affiliation:
1. ADANA ALPARSLAN TURKES SCIENCE AND TECHNOLOGY UNIVERSITY
Abstract
Bacillus subtilis has been a reliable platform for the expression of extracellular proteases for several decades. Although a majority of Bacillus subtilis subspecies express proteases, the amount of secreted enzyme varies depending on the strain and environmental conditions used. Here, two Bacillus subtilis spp. subtilis strains, NRRL B-3384 and NRRL B-3387, from the ARS Culture collection (NRRL), were compared for secreted protease activity. The highest activity was found in strain NRRL B-3384, and proteolysis occurred at temperatures as high as 80°C and across a broad range of pH, with maximum activity at pH 9.0 and 60°C indicating the presence of a thermostable alkaline protease. To our knowledge, this is the first study to evaluate protease production in Bacillus subtilis spp. subtilis strains NRRL B-3384 and B3387 and suggests that NRRL B-3384 may have utility in the production of enzymes for industrial use.
Reference71 articles.
1. 1. Jegannathan KR, Nielsen PH. Environmental assessment of enzyme
use in industrial production–a literature review. Journal of cleaner
production. 2013;42:228-40.
2. 2. Singh S, Bajaj BK. Bioprocess optimization for production of
thermoalkali-stable protease from Bacillus subtilis K-1 under solidstate
fermentation. Preparative Biochemistry and Biotechnology.
2016;46(7):717-24.
3. 3. Razzaq A, Shamsi S, Ali A, Ali Q, Sajjad M, Malik A, et al.
Microbial proteases applications. Frontiers in bioengineering and
biotechnology. 2019;7:110.
4. 4. Singhal P, Nigam V, Vidyarthi A. Studies on production,
characterization and applications of microbial alkaline proteases.
International Journal of Advanced Biotechnology and Research.
2012;3(3):653-69.
5. 5. Sharma KM, Kumar R, Panwar S, Kumar A. Microbial alkaline
proteases: Optimization of production parameters and their
properties. Journal of Genetic Engineering and Biotechnology.
2017;15(1):115-26.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献