Host Impact and Specificity of Tortoise Beetle (Cassida rubiginosa) on Canada Thistle (Cirsium arvense) in Iran

Abstract

This study determined the potential of the tortoise beetle (Coleoptera: Chrysomelidae) to control Canada thistle (Asteraceae) in Iran. Genetic analysis of the tortoise beetle, based on mitochondrial DNA, confirmed the presence of the species in Iran. A field experiment using five insect densities (0 to 20 larvae plant−1) showed a positive correlation between the number of larvae transferred and impact. Feeding by 20 larvae reduced total biomass of Canada thistle by 78% and the number of capitula by 94%. More important, when grown in competition with wheat, four and eight egg batches (corresponding to approximately 12 and 24 larvae) per Canada thistle plant increased wheat ear weight by 46 and 82%, respectively. Host range studies with 22 crop and 21 weed species using no-choice and multiple-choice tests under laboratory and field conditions and parallel data from a field survey showed that joint feeding and oviposition were restricted to Canada thistle and a few other weed species. Limited feeding, without oviposition, was recorded on an additional seven weed species but also on safflower (10 to 15% reduction in biomass), and common sunflower (< 10%); the latter only under no-choice conditions. The growing period of either crop species, however, does not coincide with the feeding period of the tortoise beetle in the field. Findings indicate that the tortoise beetle is a promising biological control agent for Canada thistle in arable crops and grasslands in Iran. Other complementary methods will likely be needed to prevent substantial yield losses.