On-Chip DNA Methylation Analysis Using Osmium Complexation-Reference-Cited by-同舟云学术

On-Chip DNA Methylation Analysis Using Osmium Complexation

Published:2011 Issue: Volume:2011 Page:1-5
ISSN:2090-021X
Container-title:Journal of Nucleic Acids
language:en
Short-container-title:Journal of Nucleic Acids

Author:

Sugizaki Kaori¹,Umemoto Tadashi¹,Okamoto Akimitsu¹²

Affiliation:

1. Nucleic Acid Chemistry Laboratory, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan

2. PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan

Abstract

The development of a reaction for detecting the presence/absence of one methyl group in a long DNA strand is a chemically and biologically challenging research subject. A newly designed chemical assay on a chip for the typing of DNA methylation has been developed. A methylation-detection probe fixed at the bottom of microwells was crosslinked with methylated DNA mediated by osmium complexation and contributes to selective amplification of methylated DNA.

Publisher

Hindawi Limited

Subject

Molecular Biology,Biochemistry

Link

http://downloads.hindawi.com/journals/jna/2011/480570.pdf

Reference25 articles.

1. Relationships between chromatin organization and DNA methylation in determining gene expression

2. Effects of DNA methylation on DNA-binding proteins and gene expression

3. Eukaryotic DNA methylation as an evolutionary device

4. Genomic imprinting in mammals: an interplay between chromatin and DNA methylation?

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